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Integration of ex-vivo precision-cut liver slice (PCLS) culture with microfluidic NMR metabolomics

Integration of ex-vivo precision-cut liver slice (PCLS) culture with microfluidic NMR metabolomics
Integration of ex-vivo precision-cut liver slice (PCLS) culture with microfluidic NMR metabolomics

We present a novel microfluidic perfusion system for liver tissue slices that allows direct characterization of the perfusion fluid by micro nuclear magnetic resonance (NMR) spectroscopy and imaging. This system enables direct non-invasive observation of the metabolic processes on the liver slice in real time. Integration of a microfluidic system with high-performance NMR spectroscopy has been achieved with careful management of sensitivity and magnetic susceptibility effects to maintain spectral resolution. The system presented here combines excellent NMR performance with the ability to sustain the PCLS over several hours of perfusion.

Metabolomics, Microfluidics, NMR, Tissue slice culture
60-61
The Printing House for Chemical and Biological Microsystems Society
Patra, Bishnubrata
3bfe5fe9-78ef-4026-9f42-2dd75cfea3b2
Sharma, Manvendra
e249236d-221d-4e59-8440-011f6863f891
Karsten, Ruby
ec456492-db81-447f-92b6-30cb51941d00
Grajewski, Maciej
5689aa46-f98e-40dd-9e01-53de85a5429d
Verpoorte, Sabeth
31dc40ec-11a0-4a66-aa48-1ac761c21baa
Utz, Marcel
c84ed64c-9e89-4051-af39-d401e423891b
Patra, Bishnubrata
3bfe5fe9-78ef-4026-9f42-2dd75cfea3b2
Sharma, Manvendra
e249236d-221d-4e59-8440-011f6863f891
Karsten, Ruby
ec456492-db81-447f-92b6-30cb51941d00
Grajewski, Maciej
5689aa46-f98e-40dd-9e01-53de85a5429d
Verpoorte, Sabeth
31dc40ec-11a0-4a66-aa48-1ac761c21baa
Utz, Marcel
c84ed64c-9e89-4051-af39-d401e423891b

Patra, Bishnubrata, Sharma, Manvendra, Karsten, Ruby, Grajewski, Maciej, Verpoorte, Sabeth and Utz, Marcel (2019) Integration of ex-vivo precision-cut liver slice (PCLS) culture with microfluidic NMR metabolomics. In 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019. The Printing House for Chemical and Biological Microsystems Society. pp. 60-61 .

Record type: Conference or Workshop Item (Paper)

Abstract

We present a novel microfluidic perfusion system for liver tissue slices that allows direct characterization of the perfusion fluid by micro nuclear magnetic resonance (NMR) spectroscopy and imaging. This system enables direct non-invasive observation of the metabolic processes on the liver slice in real time. Integration of a microfluidic system with high-performance NMR spectroscopy has been achieved with careful management of sensitivity and magnetic susceptibility effects to maintain spectral resolution. The system presented here combines excellent NMR performance with the ability to sustain the PCLS over several hours of perfusion.

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More information

Published date: 1 January 2019
Venue - Dates: 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019, , Basel, Switzerland, 2019-10-26 - 2019-10-31
Keywords: Metabolomics, Microfluidics, NMR, Tissue slice culture

Identifiers

Local EPrints ID: 445039
URI: http://eprints.soton.ac.uk/id/eprint/445039
PURE UUID: 5da6d89a-975c-4c45-a076-465aa2a172bc
ORCID for Marcel Utz:

Catalogue record

Date deposited: 18 Nov 2020 17:30
Last modified: 18 Nov 2020 17:30

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Contributors

Author:
Author: Manvendra Sharma
Author: Ruby Karsten
Author: Maciej Grajewski
Author: Sabeth Verpoorte
Author:

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